Application

Research Sub CategoryNeurochemistry & NeurotrophinsGrowth Cones & Axon Guidance

Western blot: 1-10 µg/mL using ECL. Nogo-B has a molecular weight of approximately 37 kDa (Chen et al. 2000; GrandPre et al. 2000; Goldberg & Barres 2000; Prinijha et al. 2000; Tessier-Lavigne & Goodman 2000; Nagase et al. 1998).

Immunohistochemistry: not tested. It is recommended that the antibody be tried at 2-20 µg/mL on paraformaldehyde fixed tissue.

ELISA: 1:10,000-1:100,000 using 50-100 ng control peptide per well.

Optimal working dilutions must be determined by the end user.

Research CategoryNeuroscience

This Anti-Neurite Outgrowth Inhibitor B Antibody is validated for use in ELISA, WB for the detection of Neurite Outgrowth Inhibitor B.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

A 12 amino acid peptide sequence within the N-terminus and at the splice site of Nogo-A from the putative extracellular domain of rat Nogo-B (Chen et al. 2000; GrandPre et al. 2000; Goldberg & Barres 2000; Prinijha et al. 2000; Tessier-Lavigne & Goodman 2000; Nagase et al. 1998).

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified immunoglobulin. Liquid in PBS, containing 0.1% BSA and 0.05% sodium azide.

Specificity

Recognizes rat Neurite Outgrowth Inhibitor Protein B (Nogo-B). The immunogen sequence is present only in Nogo-B and absent in Nogo-C.

SPECIES REACTIVITIES: The immunogen peptide is 83% conserved in human Nogo-B/Foocen-m/foocen-m2. Reactivity with other species has not been confirmed.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.